| 학회 | 한국화학공학회 |
| 학술대회 | 2021년 봄 (04/21 ~ 04/23, 부산 BEXCO) |
| 권호 | 27권 1호, p.493 |
| 발표분야 | 생물화공 |
| 제목 | Optimization of Enzymatic Saccharification to Improve Glucose Recovery from Spent Coffee Grounds |
| 초록 | Coffee has increased consumption and the use of disposable plastic cups has also increased, resulting in soil and water pollution. Lactic acid is a raw material for polylactic acid, a biodegradable plastic. In this study, spent coffee grounds (SCG) were used as the feedstock for Lactobacillus fermentation to produce lactic acid. Statistical optimization was performed to derive pretreatment conditions using response surface methodology. The optimum conditions for alkali pretreatment of SCG were as follows: 75°C, 3% KOH and 2.8 h. Pretreated SCG were hydrolyzed to convert into fermentable sugar for lactic acid production. The optimum enzymatic hydrolysis conditions were as follows: enzyme loading of 30 FPU cellulase, 15 CBU cellobiase and 50 MNU mannanase based on 1 g biomass and time of 96 h. Fermentable sugar was recovered 1.6-fold higher than the control group by optimizing the saccharification process. SCG hydrolysates were used for lactic acid fermentation and the conversion rate was estimated to be 55.8%. The maximum lactic acid production based on 1,000 g SCG was found to be 101.2 g, a 1.6-fold improvement compared to the control group. |
| 저자 | 이강현 1 , 이정호 1 , 김승희 1 , 박철환 2 , 유하영 1 |
| 소속 | 1 상명대, 2 광운대 |
| 키워드 | 생물화학공학 |
| 원문파일 | 초록 보기 |
