| 학회 | 한국화학공학회 |
| 학술대회 | 2022년 봄 (04/20 ~ 04/23, 제주국제컨벤션센터) |
| 권호 | 28권 1호, p.904 |
| 발표분야 | [주제 10] 헬스케어 |
| 제목 | Hierarchical topography enhancing cardiomyocytes maturation through synergistic stimulation of nano-and micro-structure |
| 초록 | Recently, the topography of biomaterials has provided an essential scaffold mimicking the niche environment. Remarkable improvements have been achieved to provide biophysical cues in micro and nano-scale, but there still remain challenges for mimicking multiscale hierarchical features of native tissues. Here, we developed the hierarchical topography combining micro-wrinkle (μW) and nano-pillar (nP). First, we observed the response of cardiomyocyte (CM) depending on the wavelength of uW, which showed two types of cellular alignment. Also, CMs were tightly attached on the low aspect ratio nPs (A.R < 0.8) and formed focal adhesion densely. Through systematical investigation, we fabricated the hierarchical structure incorporating μW and nP. On the hierarchical structures with large groove (cell width < wavelength), we found the synergistic stimulation for the cardiac maturation of H9C2 and iPSC-derived cardiomyocytes. Confined within groove, CMs could form focal adhesion intensely and advance the maturation (~ 3 times higher). This topographic effect was mediated by lamin expression. We believe this systematically designed hierarchical topography can provide new insights for multi-scale biophysical stimulation platforms. |
| 저자 | 안현아 1 , 조영학 1 , 윤근태 2 , 임성갑 1 , 정희태 1 |
| 소속 | 1 KAIST, 2 NNFC |
| 키워드 | 고분자(Polymer) |
| 원문파일 | 초록 보기 |
