| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Engineering of the substrate binding pockets of α-ketoglutaric semialdehyde dehydrogenase for enhancing the activity with regard to 3-hydroxypropionaldeyhde |
| 초록 | 3-Hydroxypropionic acid (3-HP) is a key building block for valuablechemicals. A biological route for synthesizing this molecule is two-step enzymatic reactions; dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA)by glycerol dehydratase and then oxidation of 3-HPA to 3-HP by aldehydedehydrogenase. Here, we report an aldehyde dehydrogenase, anengineered a-ketoglutaric semialdehyde dehydrogenase from Azospirillumbrasilense. The variant, named 2C10, was obtained by applying alarge ALDH library to a transcription factor based selectionmethod and then a screening method for observing the activities ofindividual clones. 2C10 exhibited a 4.65-fold higher catalyticactivity toward 3-HPA than the WT. The flask culture of Pseudomonasdenitrificans with 2C10 resulted in an approximately 30% increasein 3-HP titer compared with that obtained using WT. MD simulationssuggested that compared to the WT, 2C10 has a less flexible and smaller bindingpocket for aldehyde substrates. |
| 저자 | 박예섭 1 , abdul nasir 1 , 최선주 1 , Thuan Phu Nguyen-Vo 2 , 류희창 2 , 석주연 3 , 정규열 3 , 박성훈 2 , 유태현 1 |
| 소속 | 1 아주대, 2 UNIST, 3 포항공과대 |
| 키워드 | directed evolution ; aldehyde dehydrogenase ; 3-HP |
