| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.391 |
| 발표분야 | 생물화공 |
| 제목 | PEG Grafting on the Collagen Matrix of Islets for Prolongation of Islet Allografts Survival and Reduction of the Cyclosporine A Dosage |
| 초록 | Transplanted islets are rejected by host’s immune rejection due to the recognition as a foreign material. In this study, to prevent the rejection of islets, biocompatible polyethylene glycol (PEG) was grafted onto the collagen matrix of islets and also low dose of cyclosporine A (CsA), one of immunosuppressive durgs, was daily administered into the diabetic rats after PEG-grafted islet transplantation. The mean survival time (MST± s.e., n=7) of PEG-grafted islets and free islets was 8.3 ± 0.7 and 7.6 ± 0.6 days, respectively. When 3 mg/kg of CsA was administered for 2 weeks, the MST of PEG-grafted islets or free islets was 55.3 ± 15.8 and 12.8 ± 0.6 days, respectively. However, when 0.5 or 1 mg/kg of CsA was continuously administered after 3 mg/kg of CsA administration for 2 weeks, PEG-grafted islets of all recipients survived for more than 100 days. In the histological analysis, immune cells infiltrated into the free islets but did not infiltrate into the PEG-grafted islets. Also, insulin-positive islets were shown on the all PEG-grafted islets. Thus, these results show that the grafted PEG could significantly reduce the dose of immunosuppressive drugs and the PEG-grafted islets could survive for long period. Therefore, the PEG grafting might be a useful tool for immune suppression in vivo. |
| 저자 | 이동윤 1 , 박상진 1 , 남종희 2 , 변영로 1 |
| 소속 | 1 광주과학기술원, 2 전남대 |
| 키워드 | PEG ; pancreatic islet ; transplantation ; immune rejection ; cyclosporine A |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
