| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 가을 (10/29 ~ 10/30, 호서대학교(아산캠퍼스)) |
| 권호 | 10권 2호, p.1535 |
| 발표분야 | 생물화공 |
| 제목 | Investigation the tip-sample interaction of the AFM for biological nano scale analyses |
| 초록 | Atomic force microscope phase lag imaging of protein-DNA oligonucleotide complexes has been performed to visualize the immobilized oligonucleotides on the protein surface. In normal sample conditions, neither the topographic nor phase lag images show any discriminate signals for the immobilized oligonucleotides. Use of a highly humid incubator, controls the surface humidity. Thereby, it reveals the oligonucleotide location by the local difference of tip adhesion distribution. The resultant phase lag image shows extremely strong signals in the center of the protein surface, indicating the location of oligonucleotides. This experiment, however, demonstrates the strong adhesion force between the tip and the sample leads to the production of abnormal image artifact. In the presentation, we will show the current effort to reduce the imaging artifact by a new tip preparation method. |
| 저자 | 장상목 1 , 김종민 1 , 무라마츠 2 |
| 소속 | 1 동아대, 2 동경대 |
| 키워드 | AFM ; oligonucleotide |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
