| 학회 | 한국화학공학회 |
| 학술대회 | 2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 | 14권 2호, p.2697 |
| 발표분야 | 생물화공 |
| 제목 | Development and evaluation of various recombinant Escherichia coli strains for 3-HP production |
| 초록 | Previously, we studied the production of 3-hydroxypropionic acid (3-HP) from glycerol using the recombinant Escherichia coli expressing glycerol dehydratase (dhaB1, dhaB2, and dhaB3) and aldehyde dehydrogenase (aldH).The present study aims to improve the ability of the recombinant E. coli.In order to do so, the recombinant strains were further developed to contain the genes gdrAB, which encode the glycerol dehydratase reactivase, and the gene KGSADH-I (aketoglutaric semialdehyde dehydrogenase) in place of aldH, under two different vectors.When induced with IPTG, the specific activi ties of DhaB at co-expressed conditions decreased drastically than expressing it individually. But, the specific activities of AldH and KGSADH-I were shown a slight difference, at the co-expression conditions. The crude cell activity of KGSADH-I exhibited 2-fold higher specific activity than that of AldH. When the recombinants harboring the gene aldH or KGSADH-I with dhaB and gdrAB grown on a glycerol medium, the maximum titer of 3-HP was 14.9 mmol l-1 with AldH construct, and 31 mmol l1 with KGSADH-I construct.Here, we demonstrate enhanced production of 3-HP under shake flask conditions was closely related to the enzyme characteristics of the biocatalyst. |
| 저자 | C. Rathna Singh , 박성훈 |
| 소속 | 부산대 |
| 키워드 | 3-hydroxypropionic acid ; glycerol ; glycerol dehydratase reactivase ; 3-hydroxypropionaldehyde dehydrogenase ; protein expression ; recombinant E. coli |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
