| 학회 | 한국화학공학회 |
| 학술대회 | 2012년 봄 (04/25 ~ 04/27, 제주 ICC) |
| 권호 | 18권 1호, p.876 |
| 발표분야 | 재료 |
| 제목 | Nickel Ferrite (NiFe 2 O 4 ) Nanoparticle Clusters for the Efficient Recyclable Separation of Histidine-Tagged Proteins |
| 초록 | Affinity separation based on a specific interaction between a metal ion and histidine-tagged (His-tagged) protein is one of the most efficient methods for purifying recombinant proteins. In order to devise a simpler and more efficient method for the recyclable separation of His-tagged protein, nickel ferrite (NiFe 2 O 4 ) nanoparticle clusters (NPCs) were synthesized by a simple hydrothermal method and employed in this work. The structure of NiFe 2 O 4 NPCs provides a large surface area (105.0 m2 g-1) and pore volume (0.32 cm3 g-1), which are preferable for the separation of large amounts of proteins. The high magnetic saturation value (41.3 emu g-1) and superparamagnetic property of these materials lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were strongly maintained for up to 7 cycles. |
| 저자 | 천진녕 , 서상우 , 정규열 , 이진우 |
| 소속 | 포항공과대 |
| 키워드 | Nickel ferrite ; Superparamagnetic nanoparticles ; Histidine-tagged protein separation |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
