| 학회 | 한국화학공학회 |
| 학술대회 | 2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 | 20권 1호, p.340 |
| 발표분야 | 생물화공 |
| 제목 | Hyperthermophilic Carbonic Anhydrase: Application for CO 2 Capture System Development |
| 초록 | In order to employ carbonic anhydrases (CAs, potential biocatalysts for CO2 sequestration) under the non-natural process conditions, highly-thermostable CAs should be explored. Here, we expressed the codon-optimized sequence of PMCA, cloned from the thermophile Persephonella marina EX-H1 found in marine vents, and characterized its hyper-thermostable properties. Removal of the PMCA signal peptide (sp) resulted in the production of about 5 times more purified protein, PMCA(sp-), than from the intact gene in an E. coli expression system. PMCA(sp-) has a wide pH tolerance (optimum: pH 7.5), retained 80% of its activity after 15 min incubation at 100°C and almost 50% activity even after 2 h at 100°C. Apparent Km and Vmax for the p-nitrophenylacetate were 5.90 mM and 0.020 µmol min-1. Various metal ions were examined to enhance or inhibit activity. Finally, we demonstrated that in the presence of Ca2+, PMCA(sp-) readily catalyzed the conversion of CO2 to CaCO3 (calcite form). |
| 저자 | 백승필 , 민기하 , 유영하 , 손려강 , 여기백 , 박기성 , 전소영 , 기미란 |
| 소속 | 고려대 |
| 키워드 | Carbonic Anhydrase ; CO2 Capture ; Hyperthermophilic |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
