학회 한국화학공학회
학술대회 2014년 가을 (10/22 ~ 10/24, 대전 DCC)
권호 20권 2호, p.2479
발표분야 신진연구자 심포지엄
제목 Enzyme-based biocatalysts for CO 2 sequestration
초록 Carbonic anhydrase (CA) is an enzyme that catalyzes reversible hydration of CO 2 . It has been recently suggested that this remarkably fast enzyme can be used for sequestration of CO 2 , making this a promising alternative for chemical CO 2 mitigation. For its practical application, we developed efficient and economic biocatalysts with high stability, mainly based on a recombinant CA originated from Neisseria gonorrhoeae (ngCA).
First, we engineered ngCA in the periplasm of E. coli to promote the economical use of enzymes, thereby creating a bacterial whole-cell catalyst. Next, we developed and characterized bioinspired silica nanoparticle with recombinant ngCA autoencapsulated by the silica-condensing R5 peptide fused to the ngCA. Finally, we found and characterized thermophilic α-CAs originated from Persephonella marina and Thermovibrio ammonificans, demonstrating their remarkable thermostabilities.
저자 조병훈
소속 포항공과대
키워드 Carbonic Anhydrase ; Carbon Capture ; Biocatalyst
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