| 학회 | 한국화학공학회 |
| 학술대회 | 2015년 봄 (04/22 ~ 04/24, 제주 ICC) |
| 권호 | 21권 1호, p.382 |
| 발표분야 | 생물화공 |
| 제목 | Flexible Aptamers Binding Two Different Small Molecules |
| 초록 | Small molecules are widely used in agriculture as fungicide and herbicide, which are harmful to human health and environment. The detection of these chemicals in environment is meaningful, which should be convenient, rapid and reliable. In this study, Screening of the sensitive ssDNA flexible aptamers binding two small molecules was successfully conducted, using immobilization-free Graphene Oxide (GO)-SELEX. By adopting gold nanoparticle based colorimetric assay, we confirmed the specificity of thirteen ssDNA flexible aptamers by both UV-Vis spectrophotometer and naked eye. In addition, Isothermal calorimetry (ITC) and gold nanoparticle based colorimetric are used to obtain the affinity constant, Kd and the sensitivity of the biosensor. These ssDNA flexible aptamers could be developed as an aptasensor which provides in field point of care testing system, to detect small molecules. |
| 저자 | 김상훈 , 구만복 |
| 소속 | 고려대 |
| 키워드 | aptamer ; GO-SELEX |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
