| 학회 | 한국화학공학회 |
| 학술대회 | 2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 | 24권 1호, p.327 |
| 발표분야 | 생물화공 |
| 제목 | Accurate point-of-care diagnosis of AIDS based on label-free one-step-immunoassay |
| 초록 | We developed an accurate, rapid, simple, and label-free assay method for point-of-care diagnosis of AIDS, which quickly produces strong optical signals through one-step-immunoassay. The HIV proteins, gp41, p24, and/or gp120 were used as the probes to detect anti-HIV antibodies in AIDS patient sera. In particular, gp41 and p24 were genetically presented on the surface of engineered protein nanoparticles to prepare sensitive 3-dimensional (3D) probes. The 3D probes also present multi-copies of hexa-histidine peptide (H6) on their surface to chemisorb gold ions (Au3+), which is essential to producing strong optical signals. Point-of-care diagnostic performance of the developed one-step-immunoassay was compared with that of conventional lateral flow assay (LFA) using 30 AIDS patient sera. The sensitivity of LFA was only 63% when a single antigen (gp41) was used but enhanced to 90% when three different antigens (gp41, p24, and gp120) were used together as the assay probes. On the contrary, the one-step-immunoassay using gp41 only produced strong optical signals within 15 min without causing any false negative/positive signals, showing 100% sensitivity and 100% specificity. |
| 저자 | 권정혁 , 장영은 , 오재환 , 이지원 |
| 소속 | 고려대 |
| 키워드 | 생물화공 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
