| 학회 | 한국화학공학회 |
| 학술대회 | 2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 | 24권 1호, p.330 |
| 발표분야 | 생물화공 |
| 제목 | Scalable microfluidic static droplet arrays for biochemical analysis based on chemical concentration gradient |
| 초록 | We present a microfluidic static droplet array (SDA) device that can generate high-resolution concentration gradient. The system consists of discrete sample dispensing channel, array units, and microvalves for droplet generation and droplet storage. We applied a negative pressure on the storage chamber to increase the capacity of storage chamber. The size of droplet is controlled by valve open time, and the storage chamber can contain the droplets up to 32 with 20 milliseconds valve open time. The gradient was generated by combinatorial storage of binary concentration of droplets. We confirmed that the resolution of concentration gradient is achieved down to 1/32. We also performed enzymatic inhibition assay for IC50 using β–galactosidase and PETG, and we achieved IC50 value of 2.18 μM. |
| 저자 | 이병진 1 , 정헌호 2 , 이창수 1 |
| 소속 | 1 충남대, 2 전남대 |
| 키워드 | 생물화공 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
