| 학회 | 한국화학공학회 |
| 학술대회 | 2018년 봄 (04/25 ~ 04/27, 창원컨벤션센터) |
| 권호 | 24권 1호, p.331 |
| 발표분야 | 생물화공 |
| 제목 | Precise precursor rebalancing for hexanoic acid production by fine-tuning of atoB expression in recombinant Escherichia coli |
| 초록 | In recombinant E. coli strains, hexanoic acid is generally synthesized from artificial pathways using acetyl-CoA and butyryl-CoA as precursors. Here, we demonstrate that rebalancing of metabolic fluxes at acetyl-CoA can substantially improve the titer and productivity of hexanoic acid. First, the expression of mhact coding ACT increased hexanoic acid production by 47% compared to that with an E. coli thioesterase, TesB. Next, metabolic flux was optimized at the acetyl-CoA branch point by fine-tuning the expression level of the gene for acetyl-CoA acetyltransferase (AtoB). Four different synthetic 5′-untranslated regions (5′-UTRs) were designed for atoB using UTR Designer to modulate the expression level of the gene. The flux-optimized variant produced 528 mg/L hexanoic acid in 36 h, and this titer represents an 8.7-fold increase compared to that of the non-optimized parental strain. Notably, the productivity of the optimized strain was 14.7 mg/L/h and this value was the highest among the recombinant E. coli strains when using a similar inoculum size for fermentation. Our results show that fine-tuning the expression level of atoB is critical for production of hexanoic acid. |
| 저자 | 김성경 1 , 장성호 1 , 임재형 1 , 전병승 2 , 김정연 3 , 김경헌 3 , 상병인 2 , 정규열 1 |
| 소속 | 1 포항공과대, 2 한양대, 3 고려대 |
| 키워드 | 생물화공 |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
