TY - CHAP AU - Recio-Aldavero,J. AU - Muñoz-Moreno,L. AU - Román,I.D. AU - Bajo,A.M. KW - Culture media KW - Extracellular vesicles KW - Gelatinases KW - Matrix metalloproteinases KW - Tissue extracts T1 - Fundamentals of Zymography: Applications to the Study of Biological Samples LA - eng PY - 2025/// SP - 37 EP - 46 T2 - Methods in Molecular Biology SN - 1940-6029 VL - 2918 PB - Humana Press Inc. AB - Zymography is a very useful technique to determine the gelatinolytic activity of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9). This work describes the preparation and use of different biological samples (culture media, cell lysates, tissue extracts, and extracellular vesicles), as well as the determination of gelatinolytic activity in these samples. Proteins are separated on sodium dodecyl sulfate (SDS) polyacrylamide gels in the presence of gelatin. Once the total proteins have been resolved, specific activation of MMP-2 and MMP-9 found in the gel and staining-destination are performed to visualize this enzymatic activity. With a fast and simple method, it is possible to determine the catalytic activity of gelatinases 2 and 9 from different types of biological samples. DO - 10.1007/978-1-0716-4482-9_4 UR - https://portalcientifico.uah.es/documentos/683023bd9fdcd03a7a2940f7 DP - Dialnet - Portal de la Investigación ER -