High Performance Liquid Chromatography (HPLC) for Ampicillin and Cefuroxime

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Document Type

Poster

Publication Date

Fall 10-6-2022

Abstract

Appropriate levels of an active pharmaceutical ingredient (API) in medicines must be present in a medical product for the patient to receive therapeutic value. High performance liquid chromatography (HPLC) was used to develop a methodology to test for the API in Ampicillin and Cefuroxime drugs. Following the regulations of the Distributed Pharmaceutical Analysis Lab (DPAL) out of Notre Dame system sustainability requirements linearity, precision, accuracy/range, spike/degraded spike, limit of detection /quantitation, tailing factor, and number of theoretical plates were experimentally determined to meet the excepted standards.

Comments

The ampicillin methodology is finished and ready to be submitted to DPAL for verification. For Cefuroxime, the spikes need to be completed.

Source of Funding: Science Research Fellows program, the Larry and Lesley Stimpert Endowed Fund for Student-Faculty Research and the Student-Faculty Collaborative Research Fund.

High Performance Liquid Chromatography (HPLC) for Ampicillin and Cefuroxime

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