Hisayori Shigechi,et.al.,:Appl.Envron.Microbiology.,, Vol. 70, No. 8 p. 5037-5040 (2004)
Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using theyeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis α-amylase byusing the C-terminal-half region of -agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch. APPLIED AND ENVIRONMENTAL MICROBIOLOGY >> PDF