The No. 2 seedCycloneswill take on No. 7 Kentucky on Sunday, March 22, and all eyes are on whether star forward Joshua Jefferson will be available against the Wildcats after suffering an injury in the first round. Jefferson hurt his left ankle early into the contest against Tennessee State, and wasn't able to return to the game.
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It will be a key storyline with Iowa State trying to reach the Sweet 16 for the second time in three years. Here is the latest on Jefferson's status:
Iowa State Cyclones fans cheer against the Kentucky Wildcats during the first half in the second round of the2026 NCAA Men's Basketball Tournamentat Enterprise Center on March 22, 2026 in St Louis, Missouri.
(Jamie Squire, Getty Images)
Kansas Jayhawks cheerleaders perform before a second round game of the men's 2026 NCAA Tournament against the St. John's Red Storm at Viejas Arena on March 22, 2026 in San Diego, Calif.
(Denis Poroy, Imagn Images)
Nebraska's Berke Buyuktuncel celebrates with fans following a second-round game in theNCAA men's basketballtournament between Nebraska Cornhuskers and Vanderbilt Commodores at Paycom Center in Oklahoma City on March 21, 2026.
(SARAH PHIPPS, THE OKLAHOMAN via USA TODAY NETWORK)
Arkansas Razorbacks cheerleaders in the second half against the Hawaii Rainbow Warriors during afirst round gameof the men's 2026 NCAA Tournament at Moda Center on March 19, 2026 in Portland, Ore.
(Troy Wayrynen, Imagn Images)
VCU Rams fans react after a 3-pointer by Terrence Hill Jr. #6 of the VCU Rams in the first round of the 2026 NCAA Men's Basketball Tournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Jared C. Tilton, Getty Images)
Howard Bison cheerleaders perform during a first round game of the men's 2026 NCAA Tournament against the Michigan Wolverines at Keybank Center on Mar 19, 2026 in Buffalo, NY.
(Mark Konezny, Imagn Images)
BYU Cougars cheerleader in the second half against the Texas Longhorns during a first round game of the men's 2026 NCAA Tournament at Moda Center on Mar 19, 2026 in Portland, Ore.
(Craig Strobeck, Imagn Images)
Former North Carolina Tar Heels head coach Roy Williams is seen in the crowd against the VCU Rams in the second half of a first round game of the men's 2026 NCAA Tournament at Bon Secours Wellness Arena on Mar 19, 2026 in Greenville, SC.
(Bob Donnan, Imagn Images)
Texas A&M Aggies cheerleaders during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(William Purnell, Imagn Images)
Texas Longhorns band in the first half against the BYU Cougars during a first round game of the men's 2026 NCAA Tournament at Moda Center on Mar 19, 2026 in Portland.
(Craig Strobeck, Imagn Images)
St. John's Red Storm mascot Johnny Thunder mixes with players prior to taking the court during practice day ahead of the NCAA Men's Basketball Tournament at Viejas Arena at San Diego State University on March 19, 2026 in San Diego, California.
(Sean M. Haffey, Getty Images)
A Texas Christian University Horned Frogs cheerleader practices before the game during a first round game of the men's2026 NCAATournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Bob Donnan, Imagn Images)
The High Point Panthers fans cheer during the second half of a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Troy Wayrynen, Imagn Images)
The High Point Panthers cheerleaders react during the second half of a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Troy Wayrynen, Troy Wayrynen-Imagn Images)
The North Dakota State Bison mascot and cheerleaders pose for a photo before the game against the Michigan State Spartans during a first round game of the men's 2026 NCAA Tournament at Keybank Center on March 19, 2026 in Buffalo, NY
(Gregory Fisher, Imagn Images)
Nebraska Cornhuskers fans cheer after defeating the Troy Trojans during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(Alonzo Adams, Imagn Images)
The Wisconsin Badgers mascot performs during a time out during the second half of a first round game of the men's 2026 NCAA Tournament against the High Point Panthers at Moda Center on March 19, 2026 in Portland.
(Craig Strobeck, Imagn Images)
A view of Buddy the Street Dog as Queens University of Charlotte Royals guard Yoav Berman talks to the media during a practice session ahead of the first round of the men's 2026 NCAA Tournament at Enterprise Center in St. Louis on March 19, 2026.
(Jeff Le, Imagn Images)
The High Point Panthers band performs prior to a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
High Point Panthers fans cheer prior to a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
The Wisconsin Badgers cheerleaders preform during the first half of a first round game of the men's 2026 NCAA Tournament against the High Point Panthers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
The South Florida Bulls mascot performs during the second half against the Louisville Cardinals during a first round game of the men's 2026 NCAA Tournament at Keybank Center on March 19, 2026 in Buffalo, NY.
(Mark Konezny, Imagn Images)
The Texas Christian University Horned Frogs cheerleaders perform in the first half during a first round game of the men's 2026 NCAA Tournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Jim Dedmon, Imagn Images)
The Troy Trojans mascot dances on the floor during the second half against the Nebraska Cornhuskers during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(William Purnell, William Purnell-Imagn Images)
The UCF Knights band performs during a practice session ahead of the first round of the men's 2026 NCAA Tournament at Xfinity Mobile Arena on March 19, 2026 in Philadelphia.
(Bill Streicher, Imagn Images)
Nebraska Cornhuskers cheerleaders perform during the second half against the Troy Trojans during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(William Purnell, Imagn Images)
A Wisconsin Badgers cheerleader performs during the first half of a first round game of the men's 2026 NCAA Tournament against the High Point Panthers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
The Ohio State Buckeyes cheerleaders perform in the second half during a first round game of the men's 2026 NCAA Tournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Bob Donnan, Imagn Images)
Akron Zips fans in the first half against the Texas Tech Red Raiders during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
(Matt Pendleton, Matt Pendleton-Imagn Images)
Texas Tech Red Raiders cheerleaders in the first half against the Akron Zips during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
(Nathan Ray Seebeck, Nathan Ray Seebeck-Imagn Images)
Santa Clara Broncos fans react to game play against the Kentucky Wildcats during the second half of a first round game of the men's 2026 NCAA Tournament at Enterprise Center on March 20, 2026 in St. Louis, MO.
(Jeff Le, Jeff Le-Imagn Images)
Akron Zips cheerleaders and mascot in the second half against the Texas Tech Red Raiders during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
(Matt Pendleton, Matt Pendleton-Imagn Images)
Wright State Raiders fans celebrate after a Wright State Raiders guard TJ Burch (22) scores during the second half against the Virginia Cavaliers during a first round game of the men's 2026 NCAA Tournament at Xfinity Mobile Arena on March 20, 2026 in Philadelphia, PA.
(Bill Streicher, Bill Streicher-Imagn Images)
Hofstra Pride cheerleaders in the second half against the Alabama Crimson Tide during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
(Matt Pendleton, Matt Pendleton-Imagn Images)
Garth Noble of the Louisville pep band is amped at the 2026 NCAA Women's March Madness basketball tournament at the KFC Yum Center In Louisville, Kentucky. March 21, 2026.
(Matt Stone/Courier Journal)
Michigan head coach Dusty May high-fives players after 95-72 win over Saint Louis at the NCAA Tournament Second Round at KeyBank Center in Buffalo on Saturday, March 21, 2026.
(Junfu Han, USA TODAY NETWORK)
The Notre Dame Fighting Irish band and spirit squad cheer during the first round of the NCAA women's basketball tournament against the Fairfield Stags at Ohio State's Schottenstein Center in Columbus on March 21, 2026.
(Adam Cairns/Columbus Dispatch)
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Iowa State Cyclones fans cheer against the Kentucky Wildcats during the first half in the second round of the2026 NCAA Men's Basketball Tournamentat Enterprise Center on March 22, 2026 in St Louis, Missouri.
(Jamie Squire, Getty Images)
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Iowa State Cyclones fans cheer against the Kentucky Wildcats during the first half in the second round of the2026 NCAA Men's Basketball Tournamentat Enterprise Center on March 22, 2026 in St Louis, Missouri.
(Jamie Squire, Getty Images)
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Kansas Jayhawks cheerleaders perform before a second round game of the men's 2026 NCAA Tournament against the St. John's Red Storm at Viejas Arena on March 22, 2026 in San Diego, Calif.
(Denis Poroy, Imagn Images)
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Nebraska's Berke Buyuktuncel celebrates with fans following a second-round game in theNCAA men's basketballtournament between Nebraska Cornhuskers and Vanderbilt Commodores at Paycom Center in Oklahoma City on March 21, 2026.
(SARAH PHIPPS, THE OKLAHOMAN via USA TODAY NETWORK)
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Arkansas Razorbacks cheerleaders in the second half against the Hawaii Rainbow Warriors during afirst round gameof the men's 2026 NCAA Tournament at Moda Center on March 19, 2026 in Portland, Ore.
(Troy Wayrynen, Imagn Images)
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VCU Rams fans react after a 3-pointer by Terrence Hill Jr. #6 of the VCU Rams in the first round of the 2026 NCAA Men's Basketball Tournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Jared C. Tilton, Getty Images)
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Howard Bison cheerleaders perform during a first round game of the men's 2026 NCAA Tournament against the Michigan Wolverines at Keybank Center on Mar 19, 2026 in Buffalo, NY.
(Mark Konezny, Imagn Images)
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BYU Cougars cheerleader in the second half against the Texas Longhorns during a first round game of the men's 2026 NCAA Tournament at Moda Center on Mar 19, 2026 in Portland, Ore.
(Craig Strobeck, Imagn Images)
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Former North Carolina Tar Heels head coach Roy Williams is seen in the crowd against the VCU Rams in the second half of a first round game of the men's 2026 NCAA Tournament at Bon Secours Wellness Arena on Mar 19, 2026 in Greenville, SC.
(Bob Donnan, Imagn Images)
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Texas A&M Aggies cheerleaders during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
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Texas Longhorns band in the first half against the BYU Cougars during a first round game of the men's 2026 NCAA Tournament at Moda Center on Mar 19, 2026 in Portland.
(Craig Strobeck, Imagn Images)
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St. John's Red Storm mascot Johnny Thunder mixes with players prior to taking the court during practice day ahead of the NCAA Men's Basketball Tournament at Viejas Arena at San Diego State University on March 19, 2026 in San Diego, California.
(Sean M. Haffey, Getty Images)
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A Texas Christian University Horned Frogs cheerleader practices before the game during a first round game of the men's2026 NCAATournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Bob Donnan, Imagn Images)
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The High Point Panthers fans cheer during the second half of a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Troy Wayrynen, Imagn Images)
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The High Point Panthers cheerleaders react during the second half of a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Troy Wayrynen, Troy Wayrynen-Imagn Images)
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The North Dakota State Bison mascot and cheerleaders pose for a photo before the game against the Michigan State Spartans during a first round game of the men's 2026 NCAA Tournament at Keybank Center on March 19, 2026 in Buffalo, NY
(Gregory Fisher, Imagn Images)
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Nebraska Cornhuskers fans cheer after defeating the Troy Trojans during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(Alonzo Adams, Imagn Images)
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The Wisconsin Badgers mascot performs during a time out during the second half of a first round game of the men's 2026 NCAA Tournament against the High Point Panthers at Moda Center on March 19, 2026 in Portland.
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A view of Buddy the Street Dog as Queens University of Charlotte Royals guard Yoav Berman talks to the media during a practice session ahead of the first round of the men's 2026 NCAA Tournament at Enterprise Center in St. Louis on March 19, 2026.
(Jeff Le, Imagn Images)
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The High Point Panthers band performs prior to a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
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High Point Panthers fans cheer prior to a first round game of the men's 2026 NCAA Tournament against the Wisconsin Badgers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
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The Wisconsin Badgers cheerleaders preform during the first half of a first round game of the men's 2026 NCAA Tournament against the High Point Panthers at Moda Center on March 19, 2026 in Portland, Or.
(Craig Strobeck, Imagn Images)
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The South Florida Bulls mascot performs during the second half against the Louisville Cardinals during a first round game of the men's 2026 NCAA Tournament at Keybank Center on March 19, 2026 in Buffalo, NY.
(Mark Konezny, Imagn Images)
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The Texas Christian University Horned Frogs cheerleaders perform in the first half during a first round game of the men's 2026 NCAA Tournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Jim Dedmon, Imagn Images)
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The Troy Trojans mascot dances on the floor during the second half against the Nebraska Cornhuskers during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(William Purnell, William Purnell-Imagn Images)
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The UCF Knights band performs during a practice session ahead of the first round of the men's 2026 NCAA Tournament at Xfinity Mobile Arena on March 19, 2026 in Philadelphia.
(Bill Streicher, Imagn Images)
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Nebraska Cornhuskers cheerleaders perform during the second half against the Troy Trojans during a first round game of the men's 2026 NCAA Tournament at Paycom Center on March 19, 2026 in Oklahoma City.
(William Purnell, Imagn Images)
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A Wisconsin Badgers cheerleader performs during the first half of a first round game of the men's 2026 NCAA Tournament against the High Point Panthers at Moda Center on March 19, 2026 in Portland, Or.
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The Ohio State Buckeyes cheerleaders perform in the second half during a first round game of the men's 2026 NCAA Tournament at Bon Secours Wellness Arena on March 19, 2026 in Greenville, SC.
(Bob Donnan, Imagn Images)
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Akron Zips fans in the first half against the Texas Tech Red Raiders during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
(Matt Pendleton, Matt Pendleton-Imagn Images)
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Texas Tech Red Raiders cheerleaders in the first half against the Akron Zips during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
(Nathan Ray Seebeck, Nathan Ray Seebeck-Imagn Images)
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Santa Clara Broncos fans react to game play against the Kentucky Wildcats during the second half of a first round game of the men's 2026 NCAA Tournament at Enterprise Center on March 20, 2026 in St. Louis, MO.
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Akron Zips cheerleaders and mascot in the second half against the Texas Tech Red Raiders during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
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Wright State Raiders fans celebrate after a Wright State Raiders guard TJ Burch (22) scores during the second half against the Virginia Cavaliers during a first round game of the men's 2026 NCAA Tournament at Xfinity Mobile Arena on March 20, 2026 in Philadelphia, PA.
(Bill Streicher, Bill Streicher-Imagn Images)
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Hofstra Pride cheerleaders in the second half against the Alabama Crimson Tide during a first round game of the men's 2026 NCAA Tournament at Benchmark International Arena on March 20, 2026 in Tampa, Fla.
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Garth Noble of the Louisville pep band is amped at the 2026 NCAA Women's March Madness basketball tournament at the KFC Yum Center In Louisville, Kentucky. March 21, 2026.
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Michigan head coach Dusty May high-fives players after 95-72 win over Saint Louis at the NCAA Tournament Second Round at KeyBank Center in Buffalo on Saturday, March 21, 2026.
(Junfu Han, USA TODAY NETWORK)
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The Notre Dame Fighting Irish band and spirit squad cheer during the first round of the NCAA women's basketball tournament against the Fairfield Stags at Ohio State's Schottenstein Center in Columbus on March 21, 2026.
It's a tough break for the Cyclones as they hoped he would be able to suit up. A day prior, coach T.J. Otzelberger said it appeared unlikely he would be able to play. On Saturday, Jefferson in a boot and using a knee scooter.
"We've got so much confidence in this group and guys that can step up on a given night. Obviously when you have a guy like Joshua out, it's going to be imperative that other guys step in," he said.
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What happened to Joshua Jefferson?
Jefferson was injured less than three minutes into the first round game contest, appearing to twist his ankle as he landed after going for a layup. He was helped off the floor by support staff as he was unable to put any weight on his left foot.
He was later seen on crutches and didn't return to the game.
Jefferson is a major contributor for the Cyclones, averaging 16.6 points, 7.4 rebounds and 5.0 assists per game. He also has two triple doubles on the season.