Recent applications of isotopic labeling for protein NMR in drug discovery.
1. Introduction
2. Brief overview of “protein-based” NMR methods used in drug discovery 2.1 Theoretical background and limitations 2.2 Featured applications of the protein-based NMR approach: NMR assisted FBDD
3. Biological systems used for isotopic labeling 3.1 Escherichia coli expression systems. 3.2 Brevibacillus choshinensis 3.3 Pichia pastoris and other yeasts 3.4 Kluyveromyces lactis 3.5 Insect, plant, and mammalian cells 3.6 Cell-free systems
4. Advances in specific/selective isotope labeling techniques 4.1 AATS-labeling 4.2 Amino acid selective unlabeling (inverse labeling) 4.3 Combinatorial AATS-labeling for sequential assignments 4.4 Segmental labeling 4.5 Metabolic fractional labeling and moiety specific labeling 4.6 Chemical 13C-Me-Lys labeling 4.7 SAIL-method
5. In-cell NMR: Pharmaceutical NMR applications for cellular biochemistry and drug delivery 5.1 In-cell NMR in bacterial expression systems 5.2 In-cell NMR in Xenopus oocytes 5.3 In-cell NMR in mammalian cells